To explore network evolvability, we constructed recombinations of promoters (including regulatory regions) with different transcription or σ-factor genes in. Skip to main content. European Bioinformatics Institute. Evolvability and hierarchy in rewired bacterial gene networks. Sequencing DNA from several organisms has revealed that duplication and drift of existing genes have primarily moulded the contents of a given genome.

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Evolvability and hierarchy in rewired bacterial gene networks.

This rewlred centered standardized data allowed us to compare gene expression across plates Figure 1D. This library-reporter vector was then linearized rewieed restriction digestion using two separate enzymes to minimize library component loss due to double cutting and transformed into P.

The ability to program cellular behaviour is a major goal of synthetic biology, with applications in health, agriculture and chemicals production. Individual rewired promoter and CDS components are integrated into a single vector with a suitable heterologous reporter construct.

Evolvability and hierarchy in rewired bacterial gene networks.

We constructed a library consisting of promoters and CDSs that regulate cellular functions known to be important netwokrs protein overproduction, such as the unfolded protein response, heat shock response, carbon and nitrogen metabolism, and oxidative stress Figure 1C.

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Transformation of this expression construct into P. The clustering coefficient is a measure of how likely a node’s neighbours are to be connected with one another 41revealing how a node might facilitate localized information flow between its neighbors.


Engineering microbial phenotypes through rewiring of genetic networks Oliver P.

Evolvability and hierarchy in rewired bacterial gene networks – Semantic Scholar

CDS constructs in yeast and screened these for enhanced product synthesis. They thus form evolvabilitu logical focus for further analysis. Proceedings of the National Academy of Sciences 94 11, GFP fluorescence used as a proxy for protein expression.

B Mean production of lycopene in 40 ml cultures. These solutions may suggest that variable regulation of stress responses can lead to improvements in evollvability production. You must accept the terms and conditions.

In order to make readings comparable across plates OD normalized fluorescence signals were standardized by subtracting the mean and dividing by the standard deviation on an individual plate basis.

Evolvability and hierarchy in rewired bacterial gene networks

Sign In or Create an Account. URE2-l construct in andd enhanced expression population suggested that altered regulation of nitrogen metabolism or perception is gens in enhancing heterologous protein expression. Sequenced constructs were then rebuilt and independently transformed. Abstract The ability to program cellular behaviour is a major goal of synthetic biology, with applications in health, agriculture and chemicals production. Thank you for submitting a comment on this article.

From This Paper Figures, tables, and topics from this paper. UPC2 also enhanced lycopene production. Your comment will be reviewed and published at the journal’s discretion. Collins Annual review of biophysics Add comment Close comment form modal. Their combined citations are counted only for the first article.

Though the effect of knocking out or overexpressing a particular gene has been studied in many organisms, no study has systematically explored the effect of adding new links in a biological network.


The bacterial genes crtE, crtB and crtI were placed under the control of constitutive promoters to enable lycopene production.

This strategy is demonstrated in yeast strains that show significantly enhanced heterologous protein expression and higher titers of terpenoid production. The TOR signalling pathway controls nuclear localization of nutrient-regulated transcription factors. Mean values are for CTA Flask culture was performed in ml Erlynmeyer flasks with 40 ml culture volumes in YPD and grown for 72 h.

A global view of antibiotic resistance. The URE2 gene product of Saccharomyces cerevisiae plays an important role in the cellular response to the nitrogen source and has homology to glutathione s-transferases.

Topics Discussed in This Paper. Expression of proteins and metabolic pathways results in a highly unnatural cellular state that invokes a number of cellular stress responses, reducing the quality and quantity of desired products 67.

D Growth normalised GFP fluorescence for the rewiring library. High level expression of heterologous proteins may require the manipulation of multiple cellular processes at once, including metabolism, stress response and protein processing The dominance of nitrogen regulators in the identified rewiring solutions underscores the importance of optimizing resource allocation for synthetic manufacturing processes.